7.71 Biophysical Chemistry Techniques
For students who want to understand the benefits and caveats of biophysical techniques used to ascertain the structure of macromolecules, especially on the 3-D level. The first half of the course focuses on x-ray crystallography, the single most important technique used in determining the 3-D structure of macromolecules. Discussion of crystallographic theory is complemented with exercises such as crystallization, data processing, and model building. In the second half of the course, biophysical techniques are covered that supplement the 3-D characterization of biological macromolecules. Topics include CD spectroscopy, isothermal calorimetry, analytical ultracentrifugation, dynamic light scattering, and surface plasmon resonance (BIAcore). Theoretical principles behind the techniques are covered, applications are discussed, and students are performing practical exercises using instrumentation available at MIT. Meets with 5.78 when offered concurrently.
This class counts for a total of 12 credits. This is a graduate-level class.
You can find more information at the MIT Department of Biology: 7.71j/5.78j Biophysical Chemistry Techniques site or on the 7.71 Stellar site.
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